Isolation and identification of endophytic fungus Devriesia lagerstroem and its promoting effect on Dendrobium candidum growth
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Abstract:
Two hundred and four fungal isolates were obtained from stems and leaves of different plants.One endophytic fungal isolate 24L-4 was selected by pathogenicity test on Chinese cabbage.Isolate 24L-4 was identified as Devriesia lagerstroem based on its morphological characteristics and sequences of internal transcribed spacer and 18S ribosomal DNA.Isolate 24L-4 was inoculated on Dendrobium candidum tissue cultures and pot seedlings,respectively,with non-inoculated seedlings as the control.The results showed that the seedling height,leaf width,stem diameter and dry weigh were significantly enhanced by 33.3%,65.7%,27.3% and 45.1%,respectively,in the tissue cultures.Similar results were observed in the pot experiment with increase percentages by 104.0%,83.9% and 114.7% for the stem diameter,fresh weight and dry weight,respectively.The hyphae and microscierotia-like structures of isolate 24L-4 were found in the root epidermal and cortex of D. candidum.These results indicated that isolate 24L-4 had excellent growth promoting effect on D. candidum.
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谢玲,张雯龙,蓝桃菊,陈艳露,覃丽萍,NARISAWA Kazuhiko,刘斌. Isolation and identification of endophytic fungus Devriesia lagerstroem and its promoting effect on Dendrobium candidum growth[J]. Jorunal of Huazhong Agricultural University,2016,35(3):83-88.