The effects of 5406 antibiotic fertilizer on the soil pH,enzyme activity,fertility of soil,yield production and fruit quality under the greenhouse of a five years’ continuous cropping Capsicum annuum were studied.The results showed that after applying 5406 antibiotic fertilizer,the production,average fruit weight,Fe content of fruit,Mg content of fruit and Ca content of fruit was increased by 44.63%,23.96%,65.97%,8.56% and 2.67%,respectively.The contents of crude protein and phosphorus had no significant differences compared to the control group.Vitamin C content decreased slightly.The maximum activity increase of soil invertase,urease,and catalase was 37%,10%,8%,and 20%,respectively.The content of organic matter,alkali-hydrolyzable nitrogen,rapidly available kalium and rapidly available phosphorus was 9%,4%,3% and 33%,respectively.The soil pH value was increased as well.5406 antibiotic fertilizer can significantly improve yield production,fruit quality,and soil fertility.It has great application value.