The research was conducted to construct an infectious clone of porcine circovirus type 2 recombinant virus ZJ-R. One copy and two copies in tandem of the whole genomic sequence of ZJ-R were cloned into the pSK vector with restriction site of enzyme EcoRⅤ. The B cell epitopes of the ZJ-R virus structural protein were predicted by using biotic soft-wares. The epitope peptides were chosen, synthesized and coupled to KLH. Then the New Zealand White Rabbits were immunized to elicite the polyclonal antibodies, which were used later in immunohistochemical techniques. The results showed that the ZJ-R virus could be detected by IHC after 72 h post transfection of pSK-2 ZJ-R. In conclusion, an infectious DNA clone of the porcine circovirus type 2 recombinant virus ZJ-R was constructed successfully.