SSR(simple sequences repeat) and SRAP(sequence-related amplified polymorphism) markers were used to analyze the genetic diversity and relationship of 34 citrus germplasms in Guangxi Province including 11 wild citrus ，13 local varieties and 10 control varieties.Results showed that 22 primer pairs of SSR were selected to obtain 221 polymorphic bands with 10.1 polymorphic bands per primer pairs.11 primer pairs of SRAP were screened to obtain 215 polymorphic bands with 19.5 polymorphic bands per primer pairs.The genetic similarity of SSR and SRAP for all germplasms accessions was ranged from 0.78 to 0.96 and 0.60 to 0.92,respectively.Both SSR and SRAP can efficiently identify real citrus including the lime and lemon,the pummelo,the mandarin and assorted hybrids,the Citrus ichangensis Swingle,and the wide leather citrus.