The alcohol acyltransferase gene,named as OfAAT1,was isolated from Osmanthus fragrans‘Liuye Jingui’ flower. The full length of this gene was 1 386 bp encoding a protein of 461 amino acids.The result of qRT-PCR analyses showed that the expression of OfAAT1 was weak in young leaves and specific in the flower organs,with the highest level in the petals.The gene expression increased gradually with the opening of flowers and reached the maximum value at the stage of full bloom.During this stage,the expression of OfAAT1 had a significant circadian rhythm.Amino acids alignment andphylogenetic analysis showed that OfAAT1 contained conserved domain of the acyltransferase BAHD family,and had higher homology with PhCFAT from petunia,GmCHAT from soybean and StCHAT from potato.Combining with analyzing components of volatile esters,the OfAAT1 function was predicted to be the similar to that of PhCFAT,which catalyzes the synthesis of coniferyl acetate with coniferyl alcohol and the degradation of it to isoeugenol.Meanwhile,OfAAT1 might involve in the synthesis of fatty acid esters,the same as GmCHAT and StCHAT.It will be helpful for understanding the mechanisms of metabolism of volatile esters in O.fragrans Lour.