General transcription factors are involved in a wide range of eukaryote gene transcription.Rice TBP (TATA-box binding protein) gene was amplified by PCR.Totally 104 individual mutagenic TBP genes were obtained by random mutagenesis and introduced into wild rice ZH11(Oryza sativa spp.japonica) by Agrobacterium tumefaciens-mediated transformation.Various phenotypes were screened.In T1 and T2 generation,improved agronomic traits and tolerant to abiotic stresses were identified. One mutant with high yield and abiotic stress resistance was obtained.It is indicated that improving rice agronomic traits with general transcription machinery is workable.