Differential expression profiling of RPS27a and SDC2 genes in Sichuan white goose
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    Abstract:

    To elucidate the possible function of the RPS27a and SDC2 genes during ovarian development,relative expression of the RPS27a and SDC2 in 17 tissues and follicles of Sichuan white geese were determined by quantitative real-time PCR.The highest RPS27a expression was detected in the ovarian stroma (P<0.05) and the lowest was in the cerebellum.The highest expression of SDC2 was in the liver (P<0.05),and the lowest was in the breast and thigh muscle (P<0.05).In the ovarian hierarchical follicles,the RPS27a and SDC2 mRNA was significantly lower in the small yellow follicles (SYF) than that in the small white follicles (SWF).The RPS27a expression of F2 was significantly higher than that of F5 and F3.The SDC2 of F2 was as 2.05 folds as that of SWF,and was significantly higher than that of F5,F4 and F3.The SDC2 mRNA was not significantly different between F2 and F1.During the postovulatory,the RPS27a and SDC2 expression decreased significantly.The lowest expression of RPS27a and SDC2 was observed in POF3 (P<0.05).The RPS27a and SDC2 are expressed ubiquitously and tissue-specifically,suggesting that they may play an important role in maintaining the ovarian function and they may also play important roles in accelerating the process of ovarian development and postovulatory follicles degenerating.

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何珲,姜冬梅,马容,徐麒麟,陈咨余,康波. Differential expression profiling of RPS27a and SDC2 genes in Sichuan white goose[J]. Jorunal of Huazhong Agricultural University,2015,34(6):89-93.

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History
  • Received:May 04,2015
  • Revised:
  • Adopted:
  • Online: October 13,2015
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