The influence of different culture methods on the pathogenicity of six populations of Radopholus similis from imported ornamental plants were tested on carrot disks and with pot experiment in greenhouse. R.similis can spread virulence to Anthurium andraeanum with both long-term culture on carrot disks and rejuvenation on A.andraeanum, but the influence of the two methods on the pathogenicity of R.similes were different. The lowest incidence of root was 24.4% while the highest was 90.0%, the lowest productive factors was 0.20 while the highest was 2.80. The pathogenicity of six R.similis populations were reduced after long-term culturing on carrot disks, and can be stronger after rejuvenation on the host plant, but the pathogenicity would only be steady after continuous 2 rejuvenations.