By using eight microsatellite primer pairs,cross-species amplification and genetic diversity analysis were carried out from a total of 147 individuals among five species of Siniperca,namely S. chuatsi,S. kneri,S. scherzeri,S. undulata and S. obscura.The results showed that except for loci HW8 of S. undulate,eight microsatellite loci in the five species of Siniperca were highly polymorphic.The numbers of alleles per locus ranged from 4 to 8,and the mean allele numbers was 6.13.The average effective number of alleles of the five species was 3.58,with the average observed and expected heterozygosity being 0.75 and 0.59,respectively.The average polymorphism information content was 0.51.All these data showed that the eight microsatellite markers had high sensibility,and could be used for species identification and analysis in the sinipercine fishes.Using Nei’s genetic distance and genetic similarity coefficient,the UPGMA clustering showed that the genetic relationship between S. chuatsi and S. kneri,S. scherzeri and S. undulata were relatively closer,while genetic relationship between S. scherzeri and S. obscura was the furthest.