Culture conditions and reproductive characteristics of Aphelenchoides ritzemabosi on callus
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Abstract:
The reproductive characteristics of Aphelenchoides ritzemabosi (Schwartz) Steiner & Buhrer on carrot (Daucus carota) callus and sweet potato (Ipomoea batatas) callus were studied at 4 different temperatures and 6 different culture times. The results showed that the optimum temperatures of A.ritzemabosi on two calluses in culture were from 15℃ to 25℃ and it could not reproduce at temperatures greater than or equal to 30℃. The maximum reproduction rates of A.ritzemabosi on the carrot callus and on the sweet potato callus were 3937.50 at 20℃ for 5 weeks, 576.68 at 20℃ for 6 weeks, respectively. The reproduction rate of A.ritzemabosi on the carrot callus was higher than that on the sweet potato callus at same temperature and with the same culture time. This research provided the scientific basis for the confirmation of the reproductive cycle, life history and occurrence regularity of A.ritzemabosi, and it also provided a practical method for the systematical study of A.ritzemabosi.
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彭晓放,陈德强,王东伟,王珂,吴文佳,徐春玲,谢辉. Culture conditions and reproductive characteristics of Aphelenchoides ritzemabosi on callus[J]. Jorunal of Huazhong Agricultural University,2015,34(5):48-51.