Constructing FaAP1 expression vector of strawberry and its ectopic-expression in Arabidopsis
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Abstract:
To evaluate the function of FaAP1,an AP1 homologue of strawberry,the plant expression vector pBI121-FaAP1 was constructed and transformed into Agrobacterium tumefaciens strain GV3101.FaAP1 was transferred into Arabidopsis with floral-dip method.Three transgenic lines were successfully obtained and verified by antibiotic screening and PCR.The flowering time of transformants was over 10 days earlier than that of the wild-type.The vegetative growth of transformants was inhibited,with a dwarf plant height and less rosette leave numbers.The floral organs of the transformants were abnormal and did not produce fertile seeds.It is indicated that FaAP1 may be involved in regulating the flowering process.It will provide a basis for further studying the role of this gene in the flowering processing of strawberry.
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袁友泉,李超超,许馨月,张志宏,刘月学. Constructing FaAP1 expression vector of strawberry and its ectopic-expression in Arabidopsis[J]. Jorunal of Huazhong Agricultural University,2015,34(5):13-18.