The large EMS mutagenesis pool of sweet sorghum was initially screened out．Three mutants were identified and designated as SM83,SM197 and SM305.In general,three mutants had high biomass productions,but displayed a distinct biomass enzymatic digestibility under chemical pretreatments.As a comparison,both SM197 and SM305 mutants were determined with much high biomass enzymatic saccharification,due to its high hemicelluloses levels and relatively low cellulose and lignin contents.Despite that SM83 mutant remained a similar biomass digestion rate,it contained an increased soluble sugar level by 14.99%,compared with wild type.Hence,the sweet sorghum mutants will be used for breeding energy sweet sorghum.