To design a new gene encoding the multi-epitope chimeric antigen of sheeppox virus (SPPV) and express the chimeric gene,the dominant epitopes of ORF90,ORF112,ORF117,ORF55 and ORF134 gene of SPPV were analyzed and selected by computer software and reported references.A recombinant multi-epitope chimeric gene including SPPV six multi-epitope genes was constructed and cloned into prokaryotic expression vector pET-32.The recombinant plasmids pET32-mE were induced by IPTG.The SDS-PAGE analysis showed that the concentration of the fusion protein in E.coli protein was about 32,with a molecular weight of 41 ku.The optimal temperature,concentration of IPTG and induced time for the recombinant protein were 37 ℃,0.5 mmol/L and 4 h,respectively.The fusion protein was identified correctly by positive serum against capripoxvirus by Western-blotting,suggesting potential value of genetically engineering vaccine.