In order to prompt smut resistance levels of sugarcane varieties or clones,using mixed spores of Ustilago scitaminea Sydow collected in main sugarcane-producing areas in Guangdong Province as a inoculum.Adopt the method of combination of dipping inoculation with natural infection,smut resistance identification has been carried out to 34 sugarcane varieties or clones through new plant field experiments.The evaluation results showed that 00-319, 01-121, 00-256, 01-125 and 01-79 were highly resistant to smut (HR), accounting for 14.7%; 01-120, YT03-233 and CP93-1634 were of the resistance (R), accounting for 8.8%; YT96-86 and 99-57 were of the middle resistance (MR), accounting for 5.9%; 01-246, YT95-168, 01-160, YT91-1102, 01-244, YT00-318, CP89-2143 and CP94-1100 were of the middle sensitivity to smut (MS), accounting for 23.5%; 98-73, YT99-66, YT91-976, ROC22, NCo376, ROC20 and YT03-393 were sensitive to smut (S), accounting for 20.6%; ROC10, 97-639, YN83-88, F134, YT03-373, YT02-205, YT02-373, YT04-232 and NCo310 were highly sensitive to smut (HS), accounting for 26.5%. 