Utilizing endosperm-specific promoter can effectively improve the quality of rice in genetic engineering,and has good application value in rice seed bioreactor.Based on microarray data,a rice endosperm-specific expression gene DX35 was identified.A promoter named as DXCP35 with 1 356 bp in length of the gene was cloned from the genomic DNA of Minghui 63 by PCR.Promoter DXCP35 was fused with β-glucuronidas(GUS) reporter gene and transformed into rice callus through Agrobacterium-mediated transformation.DXCP35 was identified as an endosperm-specific promoter in transgenic rice plants by histochemical staining.Results of analyzing its 5′ end deletion showed that promoter fragment with 308 bp length was sufficient to maintain the endosperm-specific expression model.The core function areas of DXCP35 were preliminarily identified.