A liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) method was developed for simultaneously determining residues of four alkylphenols (APs) and bisphenol A in fresh fishes,shellfishes,and shrimps.The samples were ultrasonically extracted with acetonitrile,and then purified by PSA solid-phase extraction cartridge.Limits of detection were between 0.07 μg/kg and 0.30 μg/kg,and the limits of quantification were between 0.2 μg/kg and 1.0 μg/kg.The acceptable linearity was between 0.5-300.0 μg/L,and the related correlation coefficients were between 0.991 8-0.999 9.Recovery studies were carried out on spiked samples with concentrations between 5-100 μg/kg,and the average recoveries were between 60%-127%(n=6) with CVs of 2.2%-39.9%.The method was reliable,convenient and suitable for determining APs and BPA in seafood in import-export trade.