Field trials in different locations were carried out to study effects of controlled-release urea (CRU) application on the yield,nutrient uptaking and nitrogen fertilizer use efficiency of rice.The results showed that the application of CRU significantly increased the yield of rice grain by 5.3%-7.8% comparing with that of the common urea treatment (total as basal) when subtracted 25% N,and increased the yield by 2.6%-2.9% comparing with that of common urea treatment (split application).The significant quadratic function correlation was found between the rice yield and the application amount of CRU.Increasing the number of filled grains was the main factor that CRU increased the yield of rice.The results also showed that the nitrogen content in the dry matter and N nutrient uptaking of rice were all increased significantly for the treatment CRU.The application of CRU increased the nitrogen use efficiency by 17.1%-34.1% comparing with the common urea.The appropriate N application amount of controlled-release urea for rice was 102-128 kg/hm2under the target yield of 8.35-8.41 t/hm2.Controlled-release urea could not only decrease the nitrogen application amount by 14.7%-32.0%,but also reduce the fertilization time for labor saving and high efficiency.