Effects of dietary lipid levels on growth performance,activity and gene expression of lipoprotein lipase in GIFT tilapia (Oreochromis niloticus) juvenile
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    Abstract:

    A 90-day study was conducted to investigate effects of dietary lipid levels on performance,fat deposition,activity and expression of lipoprotein lipase (LPL) in GIFT tilapia (Oreochromis niloticus) juvenile,and to explore the correlation between LPL gene expression and fat deposition.315 GIFT tilapia juveniles(average weight 2.63±0.16 g) were randomly divided into three groups with three replications,and fed with isonitrogenous diets with different lipid levels of 3.7% (low-lipid group),7.7%(middle-lipid group)and 16.6% (high-lipid group) by supplementing with 2%,6% and 15% fish oil as lipid source,respectively.The results showed:(1) the hepatosomatic index (HSI),viscerosomatic index (VSI) and lipid contents in liver and muscle increased with increasing dietary lipid level(P<0.05),but there was a trends for condition factor (CF) with first increased then decreased (P>0.05); (2)with increasing dietary lipid levels, the weight gain (WG) first increased, then decreased, and feed conversion ratio (FCR) decreased(P<0.05); (3)LPL gene was expressed in liver and muscle of GIFT tilapia,while the expression level in liver was significantly higher than that in muscle (P<0.05); (4) during the 48 h post-refeeding period,liver LPL activity in high-lipid group was significantly higher than that in low-lipid and middle-lipid group at 6 h,but significantly lower than that in low-lipid group at the 12 h,24 h and 48 h.There was a trend in low-lipid and middle-lipid group that liver LPL activity increased first and then decreased; (5) the expression of LPL was significantly higher in high-lipid group than that in low-lipid group (P<0.05); (6) there were a significant linear positive correlation between HSI,lipid contents in liver and muscle,and liver LPL expression in GIFT tilapia juvenile.The results indicated that LPL expression in GIFT tilapia was in a tissue specific pattern and liver was the main organ for secretion and expression of LPL; high dietary lipid level could inhibit growth of GIFT tilapia juvenile,decrease feed conversion ratio,induce the expression of LPL in liver,and promote fat deposition in liver and muscle; liver LPL expression was involved in fat deposition of GIFT tilapia juvenile.

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王爱民,杨文平,於叶兵,吕富,吴文静,封功能,王资生. Effects of dietary lipid levels on growth performance, activity and gene expression of lipoprotein lipase in GIFT tilapia (Oreochromis niloticus) juvenile[J]. Jorunal of Huazhong Agricultural University,2014,33(2):96-102.

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  • Received:December 31,2012
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