Forty-seven Misgurnus anguillicaudatus microsatellite markers were randomly selected from DDBJ database and used for cross-species amplification in a population of Paramisgurnus dabryanus from Hubei Province.Forty-four pairs of primers amplified P.dabryanus DNA,while 3 primers including Mac31,Mac63 and Mac541 failed.Using genomic DNA of selfing offsprings juveniles of M.anguillicaudatus and P.dabryanus as templates,PCR amplifications were performed using three primers of Mac31,Mac63 and Mac541.The results showed that target bands were detected only in selfing offsprings of M.anguillicaudatus,suggesting that markers of Mac31,Mac63 and Mac541 are useful for identifying M.anguillicaudatus and P.dabryanus at the juvenile stage.