Eulaliopsis binata, a newly found apomictic species belonging to Poaceae,was proven to have diverse degree of apomixis among populations as well as individual.A microsatellite-enriched library was constructed by capture with streptavidin coated-beads magnetic beads adsorption.Twentyseven pairs of polymorphic SSR primer were developed.Genetic diversities of 11 populations and 71 individuals of E.binata were analyzed by 8 SSR primers producing 123 alleles.Each SSR marker amplified 6-34 polymorphic fragments.The eleven populations of E.binata had a genetic similarity coefficients ranging from 0.70 to 0.97 and could be classified into 4 clusters,indicating the most populations of E.binata had high similarity consistent with its apomictic reproduction mode.By UPGMA analysis,71 E.binata individuals could be classified into 3 groups consistent with results of population classification.However,three individuals from Xingzi population and one individual from Yangxian population were deviated from their own clusters of population respectively and nested in a different location,indicating that the genetic diversity was appeared in different individual of same population and the genetic difference may be reflected the varying degrees of apomixis of plant.