Using cultivar ‘Hongdeng’ in vitro shoots as material,the in vitro regeneration system from micro shoot-tip of sweet cherry were established via optimizing the corresponding factors.The result showed that Murashige and Skoog macro-elements (MS) supplemented with the combination of 0.3 mg/L TDZ and 0.1 mg/L IBA had the best regeneration effect from the micro shoot-tips with the size of 1.0-1.5 mm,and the survival rate of the shoot-tips could reach to 78.3% after 30 days culture.Fifty days after the subculture under the same media,the multiplication efficiency was as high as 3.9,and the shoot height might get to 8.9 mm.The newly established system may facilitate the culture of virus-free plant in sweet cherry.