Seedling cotyledons of the soybean cyst nematode （Heterodera glycines Ichinohe）-susceptible cultivars of Zhonghuang 13, Heidou 44 and Heidou 51 were co-cultured with Agrobacterium rhizogenes strain K599 under dark conditions. Transformation efficiency was tested through GUS staining.The results showed that there were significant differences among soybean genotypes in their susceptibility to A.rhizogenes. The conditions of 25 ℃ and 12 h/d light were found to be optimal for hairy root induction of Heidou 44.The transformation efficiency of Heidou 44 had no significant difference with that of Heidou 51, while it was significantly higher than that of Zhonghuang 13. Light or darkness had no effect on Zhonghuang 13, but the inducing efficiency of Heidou 44 and Heidou 51 in the dark conditions were significantly higher than that in light condition.The infective efficiency of Heidou 44 was significantly higher than that of Heidou 51 and Zhonghuang 13.