To elucidate the function of MYB in Actinidia chinesis ‘Hongyang’ pigmentation,a novel MYB gene(AcMYB) was isolated from that cultivar by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE).The full-length cDNA of AcMYB is 962 bp with an open reading frame(ORF) of 666 nucleotides encoding a protein of 221 amino acids with two typical MYB DNA binding domains at its N-terminal.The result of Blast X showed that AcMYB had high similarity with MYB transcription factors in Petunia hybrida Vilm., Vitis spp., Solanum lycopersicum L.,Antirrhinum majus L.and sequences from other plant species related to the elevation of anthocyanin pigmentation.Real-time PCR analysis indicated that the expression of MYB was positively correlated with the main anthocyanin concentration in Actinidia chinesis ‘Hongyang’ and both of them maintained high levels at the stage of pigmentation,suggesting that AcMYB may play an important role in regulating anthocyanin biosynthesis.