The shrimp were kept in tanks with recirculated seawater at a salinity of 3.3%.The water temperature was maintained at (29.0±1.0)℃.Hemolymph,hepatopancreas and muscle were sampled after administration of sarafloxacin hydrochloride at single dose of 30 mg/kg and the residue concentration of sarafloxacin hydrochloride analyses was performed by RP-HPLC.The results showed that,the residue concentration of sarafloxacin hydrochloride in hepatopancreas was higher than hemolymph and muscle.The residue concentration of hemolymph and muscle were very low and the former is higher than the latter.The elimination rate was markedly different in hemolymph，hepatopancreas and muscle.The elimination half-life of sarafloxacin hydrochloride was the shortest in muscle among the three tissues and the longest in hemolymph.