Gene expression of oilseed rape BN10 induced by methyl jasmonate and oxalic acid
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    Abstract:

    Using real-time quantitative PCR, the BN10 gene expression in roots, stems and leaves of rape was detected. Its expression after the treatment with methyl jasmonate (MeJA) and oxalic acid (OA) was also analyzed. The results indicated that the expression of the BN10 gene was detected in roots, stems and leaves of rape with the highest expression level in roots, whereas the lowest expression level in leaves. The expression level in the antiviral variety Suyou No.1 was higher than that in the susceptible variety Zhongshuang No.9. The BN10 gene expression was induced by MeJA and OA in a similar mode. BN10 was significantly induced after the treatment with OA and MeJA for 12 h and 24 h in Zhongshuang No.9 respectively. The expression level was 2.50-folds and 2.58-folds higher than that before the induction, followed by a decrease. After induction for 72 h, the gene expression level in the treatment of OA was 75% lower than that of the untreated control, whereas was still 1.13-folds higher in the treatment of MeJA than that of the untreated control. The BN10 gene expression in Suyou No.1 was similar to that in Zhongshuang No.9 induced by MeJA and OA excepted for that its expression level was higher than that in Zhongshuang No.9. BN10 was significantly induced after the treatment with OA for 6 h. The expression level was 3.05 folds higher than that before the induction, followed by a decrease.

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肖春芳,侯明生,蔡丽. Gene expression of oilseed rape BN10 induced by methyl jasmonate and oxalic acid[J]. Jorunal of Huazhong Agricultural University,2012,31(5):593-597.

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  • Received:December 30,2011
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