Five pairs SSR primers was selected to identify 90 offsprings obtained from crossing HB pomelo(Citrus grandis Osbeck) with Jiangxi seedless Ponkan(C.reticulate Blanco).The rate of true hybrid was 97.8%.There were lost and novel bands in some of the hybrids.The genetic relationship between parents and offsprings was analyzed with UPGMA.The hybrids could be divided into four groups with similarity coefficient of 0.720 and only few progenies were clustered with parents.These created germplasms provided new materials for the citrus cultivar improvement.