Effects of 24-epibrassinosteroids(EBR) on the seed germination and seedling growth of pepper(Huajiao 15) under the low temperature stress were investigated by both soaking method and addition method.The results showed that 0.001-1.000 mg/L EBR had no significant effect on the germinability of pepper seed,0.001-0.100 mg/L EBR significantly promoted the germination rate of pepper seed with soaking method at 0.050 mg/L with the germination rate of 44.00%.The content of EBR ranging from 0.001 mg/L to 1.000 mg/L had inhibition on the germination rate of pepper seed under addition method at 1.000 mg/L.Under both soaking method and addition method,the content of EBR ranging from 0.001 mg/L to 1.000 mg/L significantly inhibited the seedling root length of pepper seed with the highest inhibition rates at 1.000 mg/L,respectively.It also significantly promoted the seedling height of pepper seed under soaking method with 1.82 cm of seedling height,0.62 cm higher than that of control at the concentration of 0.050 mg/L,but under addition method EBR had no significant effect on the seedling height of pepper seed.Under soaking method,the content of EBR ranging from 0.001 mg/L to 1.000 mg/L had no significant effect on green weight and dry weight of pepper seedling.But there was no regularity under addition method.