Phosphomannose isomerase (PMI) gene from Saccharomyces cerevisiae was cloned by PCR,and transformed into Arabidopsis via floral dip method.The seeds were collected and germinated on selecting MS medium which mannose concentration was 1 g/L.Four positive plants were obtained.PCR for genome DNA and RT-PCR for RNA proved the PMI gene had integrated into the genome of Arabidopsis and was transcribed.The intron of yeast pmi was removed successfully in Arabidopsis.GUS activity also was detected in the transgenic plants. All the results means PMI gene from yeast can be used as a selectable marker in Arabidopsis transformation.