The identification of the members of a major facilitator superfamily（MFS） may provides more valuable information for the research of on the cross-resistance of insects. Illumina’s Solexa sequencing technology and the method of real-time PCR were used for cDNA identification and expression profiles analysis. A member of major facilitator superfamily (PsMFS1) was successfully obtained. Its cDNA contained 1 224 bp open reading frame (ORF) encoding 407 amino acids. The putative protein contains a drug efflux system protein domain and a major facilitator superfamily domain. Gene expression profiles analysis showed that the PsMFS1 in head, midgut and production system were relative more highly expressed than that in antenna and legs. MFS possibly plays a role in the cross-resistance of P.striolata to pesticides and its function and mechanism in vivo needed more study in the future.