Cytological observations of anther in a novel cytoplasmic male sterile line and its maintainer line Yuyou of purple Cai-tai (Brassica campestris L.ssp.chinensis L.var.utilis Tsen et Lee) was made by means of paraffin section to determine the characteristics of anther abortion.The results showed that the abortion occurred at the early stage of single-nucleus microspore.The abnormal tapetum cells were observed and microspores at early stage were extruded.In the end,the tapetum cell and microspore at early stage disaggregated.