Bacterial fruit blotch (BFB) of cucurbits, caused by Acidovorax avenae subsp.citrulli(Aac),is one of the most devastating diseases of watermelon and muskmelon. Fewer studies were done on its survival time in different places. In this study, the survival time of Aac inoculated with the anti-fampicin strain RifAac in seed,soil debit and water was investigated by isolation, bio-PCR and PCR. The results showed that Aac could survive in the watermelon seeds for 4-5 months, in the water for 2 months,in the soil for 8 months and within the soil with debris for longer than one year.