The kanamycin-resistant labeling strain Y2-pMarA, which has a good stability of genetics, was gained by electrotransformation of shuttle plasmid pMarA into Bacillus amyloliquefaciens subsp.plantarum B9601-Y2. Chinese cabbage was inoculated by drenching, coating seeds, soaking root and tobacco by drenching. The results show as follows: LB agar plate containing 10 μg/mL of kanamycin and PCR proved that Y2-pMarA could colonize in rhizosphere soil, root surface and inner root. After seeding for 37 d,the Chinese cabbage,under the natural soil condition,colonization numbers of rhizosphere soil, root surface,and inner root of natural soil were 94.31%, 95.00% and 84.75% of seeding for 7 d respectively; the corresponding ratios in sterilized soil were 75.26%, 84.92% and 177.74% respectively. In the Chinese cabbage with coating seeds,the ratios of colonization numbers of rhizosphere soil, root surface, and inner root of natural soil were 94.44%, 95.27% and 61.06% of seeding for 7 d respectively,while they were 75.6%, 90.9% and 69.1% in sterilized soil respectively. By transplanting Chinese cabbage seedlings soaked for 10 min, Y2-pMarA strain could expand into the soil and enter the roots of the plant. After transplanting for 33 d,the colonization density in the rhizosphere soil, root surface and inner root in the natural soil were 6.28×105, 9.54×105 and 4.69×103 cfu/g respectively; while the corresponding density were 6.97×105, 1.12×106 and 1.02×104 cfu/g in sterilized soil respectively.