The diversity of plant species of Wulipo Nature Reserve of Wushan County,Chongqing City was investigated by means of sampling,photo-taking,identifying and analyzing statistically.The results showed that there are 2 379 species (including infraspecific taxon) of spermatophyte belonging to 796 genera and 156 families in.At the generic level,temperate elements were the dominant components,in which temperate genera and tropical genera contributed 60.51% and 34.50% of the total (excluding cosmopolitan genera),respectively; disjuncted genera contributed 20.35% of the total (excluding cosmopolitan genera),in which East Asia and North America disjuncted genera contributed majorly.There are 37 genera endemic to China,contributed 4.99％ of the total (excluding cosmopolitan genera).Wulipo Nature Reserve has a special flora,which due to its unique location and entironment,that is very important to the study on middle China and south-west China flora.Compared with the flora of Sichuan and two neighboring areas,the flora of Wulipo Nature Reserve showed high similarity with Houhe Nature Reserve,exhibiting strong temperate elements.