Through flask-shaking fed-batch fermentation,the optimum system of glucose feeding for poly(γ-glutamic acid) (γ-PGA) fermentation by Bacillus licheniformis WX-02 was summerized as follows：the initial concentration of glucose was 70.0 g/L,then 15.0 g/L of glucose was added at 15th hour of fermentation carried out in a 3.0 L fermentor.The results showed that the biomass and the yield of γ-PGA were 3.9 g/L and 44.5 g/L,increasing 56% and 50% compared with the control.The utilization rate of glutamic acid in the end of fermentation was 46%,while that of control was 30%.Feeding glucose at the 15th hour of fermentation process could increase biomass,enhance utilization of glutamic acid and finally increase the γ-PGA yield by B.licheniformis WX-02.