In order to analyze the variation of loading in the process of shear and whether different loading rates have an impact on shear strength and shear energy or not, four different species of wheat stalks in mature stage and dried under natural conditions were selected, which were Yibi No.1, Aikang 58, Zhoumai 22 and Yumai No.7, and the stems of their second, third and fourth internodes were chosen for study. The middle of each internode was tested for cross striations shear on the TMS PRO type Texure Analyzer produced by the U.S.FTC company. Through the shear testing, the biomechanical properties of the maximum loading, shear strength and shear energy were measured for various kinds of wheat stalks on different internodes with different speeding rates. The study result indicates that the variation of loading in the process of shear was that the loading increased at the beginning, then reduced because of its structural features, then added rapidly until to rupture. Meanwhile, the hardness, shear strength and shear energy on different internodes of the tested wheat stalks were respectively within the ranges of 37.3~191.0 N, 4.2~9.8 MPa,and 43.53~432.23 mJ. As for the shear strength and shear energy on different internodes of the same species of wheat stalks, those on the second internode is the largest, the fourth is the least. At last the significance test for shear strength and shear energy was run by applying SPSS, the result shows that different speeding rates have no significant impact on shear strength and shear power.