Five experimental diets for gibel carp,Carassius auratus gibelio,were formulated to contain various percentages of common rapeseed meal (CRM) or double low rapeseed meal (DLRM) as a partial replacement for soybean meal.RM0(with 0% CRM and DLRM) was formulated as the control group.DLRM50 (CRM50),DLRM100(CRM100) were formulated to isonitrogenously replace 50% and 100% of soybean meal protein in RM0 with DLRM (CRM),respectively.The non-specific immune parameters including phagocytic activity (PP,PI) of blood leucocytes and head kidney phagocytes,serum lysozyme activity,serum complement concentrations of C3 and C4 were evaluated at every 14-day intervals.During the initial 28-day period,only PP of blood leucocytes in CRM100 group was significantly lower than that in RM0 treatment(P<0.05).From the 42th day on,both PP and PI of blood leucocytes and head kidney phagocytes in DLRM100 and CRM100 treatments were all significantly lower than those in RM0 treatment (P<0.05).On the 56th day,serum lysozyme activity in CRM100 and DLRM100 groups was significantly lower than that in RM0 group(P<0.05).From the 42th day,serum complement concentrations of C3 or C4 in DLRM100 and CRM100 treatments were significantly lower than those in RM0 group(P<0.05).The immune parameters in DLRM50 and CRM50 treatments were not significantly changed compared with those in RM0 group (P>0.05).However those in CRM50 group gradually reduced with time.We concluded that the effects on non-specific immune function might cause by antinutritional factors such as glucosinolates,sinapin,phytic,tannin,crude fibre in the rapeseed meal.