TPCR was used to amplify the high-frequent variable regions of AtCesA family genes,and then the PCR products were cloned into vector pGEX-4T-3,respectively.The fusion proteins composed of AtCESAs proteins and glutathione S transferase (GST-AtCESAs) in E.coli JM109 were induced by IPTG.The GST-AtCESAs were purified using GST affinity chromatography,and polyclonal antibodies were prepared.Western-blotting analysis showed that specific bands of six antibodies Anti-CESA1,Anti-CESA3,Anti-CESA6,Anti-CESA2,Anti-CESA5 and Anti-CESA8 could be obviously detected in the plasma membrane of Arabidopsis thaliana, whereas cross-reaction bands of antibodies Anti-CESA7 and Anti-CESA4 were occurred,suggesting that the two antibodies were failed for further research.Preparation of Anti-CESAs antibodies is good for future research on mechanism of cellulose synthesis.