In order to elucidate the molecular mechanism of Agrobacterium-mediated genetic transformation and improve the efficiency of Agrobacterium-mediated rice transformation by molecular biological methods,we studied the OsVIP1 protein function with the RNA interfering technology using the rice variety Zhonghua 11 as the host for genetic transformation.A rice protein sequence homologous to AtVIP1,named OsVIP1 was obtained by homologous searches.Two RNAi expression vectors,pDS1301-2-R1 and pDS1301-2-R2,were constructed with two fragments (R1 and R2) of the OsVIP1 cDNA,respectively,and used to transform rice variety Zhonghua 11 callus through Agrobacterium.The statistical results showed that formation of the transgenic resistant callus of pDS1301-2-R1 and pDS1301-2-R2 vectors was inhibited to some extent.PCR experiments indicated that R1 and R2 fragments were successfully integrated into the transgenic rice genome.Semi-quantitative RT-PCR analysis showed that the OsVIP1 gene expression was suppressed successfully in some RNAi transgenic rice plants.