Cloning and expression analysis of two PLT genes promoters in rice
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    Abstract:

    To study the expression and function of PLT in rice,two PLT genes in rice(OsPLT7 and OsPLT11) were firstly predicted from searching the homology of Arabidopsis PLT genes protein sequences against rice database.According to the predicted promoter sequences of OsPLT7 and OsPLT11,we designed the PCR primers and amplified the consequent promoter fragments of OsPLT7 and OsPLT11 using genomic DNA of Oryza sativa L. Zhonghua 11 as templates.Finally,we cloned these fragments into the vector containing reporter genes GUS/YFP and successfully transformed these promoter-reporter fusion vectors into rice.Gus staining observation of T1 transgenic seedlings of OsPLT7 and OsPLT11 promoter-reporter fusion vectors indicated that both OsPLT7 and OsPLT11 are expressed mainly in stem cell niche and stele area in rice root,which are very similar to the expression profile of their homologous genes in Arabidopsis.These results will be useful for the functional studies of the PLT family transcription factors.

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张 晨,王利凯,包 亮,龙 湍,陈春丽,须 健. Cloning and expression analysis of two PLT genes promoters in rice[J]. Jorunal of Huazhong Agricultural University,2012,31(2):147-151.

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  • Received:May 18,2011
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