The virulence of Meloidogyne incognita and Fusarium oxysporum f.sp.cubense race 4 (Foc 4) complex in pot experiments was measured on two Foc 4-susceptible banana cultivars (Baxi and Musa paraolisiaca) and one Foc 4-resistant banana cultivar (Nongke No.1) by inoculating simultaneously and sequentially in greenhouse. Results showed that fusarium wilt severity was significantly reduced when the nematode was inoculated before the fungus, and increased when the two pathogens were inoculated simultaneously or the fungus was introduced 5 days before the nematode. The final number of M.incognita was reduced in all the banana cultivars inoculated with the two pathogens. The fungus inoculation before the nematode and concomitant inoculation had a better suppression of nematode population. In Foc 4-resistant cultivar, resistance was stable in all the tested except for those inoculated with the two pathogens simultaneously. 