To investigate the spatial-temporal patterns and other characteristics of anther abortion in 0912A as a leaf mustard cytoplasmic male sterile line and its maintainer line 0912B,cytological observations of anther development were made by means of paraffin sections.The results showed that the abortion of male sterile line was diverse.The abortion mainly occurred at the differentiation stage of archesporial cell,which lacked the formation of pollen sacs.Part of anther abortion occurred at the period from pollen mother cell to the mono-nuclear microspore:some pollen mother cells gathered and disintegrated gradually after vacuolization; some pollen mother cells disintegrated due to the hypertrophy of tapetal layer cells in the radial direction; some pollen mother cells could develop to tetrad,then disintegrated; part of the pollen mother cells could develop to the mono-nucleus microspores,however the mono-nucleus microspores broke when they were highly vacuolated.The stamens of 0912A were also found to degenerate as abortive filaments.
邹瑞昌,万正杰,徐跃进,杨文杰,傅廷栋. Anther development of 0912A as a novel cytoplasmic male sterile line of leaf mustard[J]. Jorunal of Huazhong Agricultural University,2012,31(1):44-49.