Using ion channel protein Pollux-C containing RCK domain as a bait,14 yeast colonies were screened from Lotus japonicus AD-cDNA library with yeast two-hybrid technique.Six positive clones were found to encode elongation factor 1A (EF1A) of translation through DNA sequencing and BLAST against NCBI database.The fusion tag protein of Pollux-C and EF1A expressed in E.coli were purified.The intra-cellular domain of Pollux interacted with the C-terminus of EF1A was verified by pull-down and western blotting in vitro.The results might lay a foundation for further researches on the regulating mechanism and biological function of EF1A in symbiosis signaling in legume.