Cylindrocladium parasiticum was a very important invasive alien species in China. The biological characteristics,such as mycelial growth and sporulation,etc. of C.parasiticum were observed. The results showed that C.parasiticum could grow in different media,such as Potato Dextrose Agar (PDA), V8 juice (V8), Yeast Peptone Dextrose Agar (YPDA),Oatmeal Agar (OMA),and Synthetic Fungal(SF). Among them,OMA was most suitable for mycelial growth, whereas V8 was most suitable for microsclerotium, conidium, and perithecium formation. C.parasiticum could utilize carbon sources, such as dextrose, fructose, sucrose, maltose, lactose, and starch for growth. Among them, dextrose, lactose and starch were most suitable for mycelial growth and microsclerotium formation. However, all of the above carbon sources were unsuitable for conidium and perithecium formation. In the case of nitrogen sources,C.parasiticum could utilize sodium nitrate,urea,ammonium sulphate,yeast,and peptone for growth. Among them, sodium nitrate was most suitable for mycelial growth and microslerotium formation,whereas,urea was most suitable for conidium formation. However,perithecium was sparsely formed only in sodium nitrate. C.parasiticum could grow under temperature condition between 10-30 ℃. The optimum temperature for both of growth and conidia sporulation was between 25-30 ℃. Although the mycelial growth of C.parasiticum was not impacted by period the light,but a12 hours alternation of light and dark was suitable for conidia sporulation. C.parasiticum could grow between pH values of 4.0-10.0. But the favorable pH value for mycelial growth was neutral to partial acid.