The ultrastructural characteristics of root,stem,leaf,pollen in three kinds of Tengcha (Ampelopsis grossedentata) coming from the province of Jiangxi,Hunan,Hubei in the same ecological environment were investigated using light microscopy (LM) and scanning electron microscopy (SEM).The results showed that the cross section of root and stem had little difference among the three kinds of Tengcha,the parenchyma cells of root and stem contex all existed calcium oxalate needle crystal.There were some nonglandulars hairs in the stem epidermal cell of Hubei and Jiangxi Tengcha,calcium oxalate needle crystal also existed in the mesophyll tissue and the parenchyma cells of leaf midrib.The characteristics of lower epidermal surface of leaf including nonglandular hairs,the stripe number of the outer stomatal rim,the density,length and open of stoma were different; there were much nonglandulars at upper epidermal surface along leaf midrib,but the density and shape were different among the three kinds of Tengcha; the characteristics of upper epidermal ornamentation were different as well,the upper epidermal surface existed granular or flaky waxiness at Jiangxi Tengcha leaf,pinshripes at Hunan Tengcha leaf,but nothing at Hubei Tengcha leaf; the antheral characteristics were the same but large.The pollens of Hubei and Jiangxi were spheroidicity but pollen of Hunan Tengcha was global and the largest.The results described the special ultrastructural characteristics of the three kinds of Tengcha and the difference maybe result from its genetic diversity.