Channel catfish virus was propagated in the channel catfish ovary cell line and then purified by ultracentrifugation.This purified virus was used to immunize the female BALB/C mice to gain polyclonal antibody.ELISA was used to detect the antibody titer.The bioactivity was identified by indirect immunofluorescent assay and serum neutralization tests.The ELISA titer of the polyclonal antibody was 1∶25 600.The polyclonal antibody showed high specificity at the indirect immunofluorescent assay.It could stop the virus from infecting the cell efficiently.