Abstract:In order to investigate the highly repetitive (HR) DNA sequences among Equus asinus genome,Cot0-Cot0.1 DNA of E.asinus genome was separated and purified through two steps of hydroxypatite chromatography.Then the HR Cot library of E.asinus was constructed by gene cloning and sequencing.After the analysis and screening of the library,18 repetitive elements were identified and submitted to GenBank,including 4 LINEs,4 SINEs,3 satellites,4 simple repeats,1 LTR retrotransposon and 2 unknowns.