The acute toxicity of representative heavy metals (copper and cadmium),polycyclic aromatic hydrocarbons (phenanthrene and naphthalene) and pyrethroid pesticide (deltamethrin and cypermethrin) to White Cloud Mountain minnow Tanichthys albonubes were studied by using the static-test method in the laboratory.The results showed that,the 96 h-LC50 for copper and cadmium to the minnow were 0.054 and 4.610 mg/L,respectively; copper was a drastic toxicant and cadmium was a high toxicant to the minnow.And the safe concentrations of these metals were 0.005 and 0.461 mg/L,respectively.The 96 h-LC50 for phenanthrene and naphthalene to the minnow were 0.913 and 10.849 mg/L,respectively. Phenanthrene was a drastic toxicant and naphthalene was a medium toxicant to the minnow. And the safe concentrations of these PAHs are 0.091 and 1.085 mg/L,respectively.The 96 h-LC50 for deltamethrin and cypermethrin were 1.217 and 6.256 μg/L,respectively.Deltamethrin and cypermethrin were both drastic toxicants to the minnow.And the safe concentration of them are 0.122 and 0.626 μg/L,respectively.The minnow was very sensitive to these three kinds of pollutants and it could be used as a biological agent for evaluating and monitoring relative water pollution.