In this study, OBP from Bactrocera cucurbitae was cloned and named as BcucOBP. The full-length of open reading frame（ORF）is 447 bp, encoding 149 amino acid residues. Amino acid sequence alignment and 3D-Homology Modeling analysis showed that BcucOBP shared the typical structural feature of OBP with other insects, including 6 conservative Cys residues and 6α-helixes in the sequence. A recombinant plasmid pET28a (+)-BcucOBP was constructed and transformed into Escherichia coli BL21（DE3）. Western blotting indicated that the His-tagged BcucOBP stably expressed in DE3 induced by IPTG. Real-time PCR analysis shows that BcucOBP mRNA is expressed nearly in all tissues except for female visceral segment. The relative concentration of BcucOBP mRNA is the highest in antenna of B.cucurbitae compared with other tissues. BcucOBP mRNA shows clear gender-differential expression pattern in general section and the foreleg. The special expression pattern suggested that BcucOBP not only acted as a general odor protein but also was involved in the transport of pheromone in the mating behavior of B.cucurbitae. 