A new method for eradicating the double infected pathogen of CTV and CTLV incitrus was explored in this experiment.The citrus plants were selected from those conserved in net greenhouse of National Indoor Conservation Center of Virus-free Germplasms of Fruit Crops,Huazhong Agricultural University.The infection of pathogen was detected with reverse transcripts PCR (RT-PCR).Results showed that the suitable protocol was using the treatment of the antiviral medicament with Chinese medicines (eight times) and combinated with heat treatment of the highest temperature at 40℃ during the day(6 h/d),the temperature at 25℃ in the evening kept for fifty days in the PC made greenhouse.The protocol mentioned above was proved to completely eradicate double infected pathogen of CTV and CTLV; Furthermore,the combined treatment of shoot-tip grafting and the antiviral Chinese medicines was identified 85.7% eradication of the double pathogen.In above experiments,the leaves were sampled at 1,3,5,7,9 d after the Chinese medicines treatment.The results showed that the activity of the resistance related enzymes PPO,POD,SOD were increased compared with the control.