The antifungal substance produced by Bacillus amyloliquefaciens WH1,isolated from the root of rice,was identified with infrared spectra.The inhibitory effect of WH1 towards Colletotrichum gloeosporioides was measured by suppression tests both in the plate and leaves in vitro.The results showed that the antifungal substance WH1 produced was cyclic lipopeptide,the fermentation broth was detected with the activity to deform the mycelium of C.gloeosporioides,and the highly diluted fermentation broth still had a significant control effect in vitro.In order to make WH1 a dedicated formulation to control C.gloeosporioides,the fermentation condition of WH1 was optimized by Plackett-Burman design and Taguchi array design.After optimization,the lipopeptide production has increased significantly.Keeping the dissolved oxygen above 30%,the fermentation in fermentor of 3 L,20 L and 500 L was scaled up successfully.Tenfold diluted fermentation broth of fermentation in 500 L fermentor has inhibitory rate of 50.8% on C.gloeosporioides,and still kept inhibitory rate of 86.2% on fungus after spray drying.